NSR1000 Super-Resolution Microscope

By combining SIM super-resolution and confocal imaging on the NIB1000 research-grade inverted microscope,the NSR1000 enables multi-dimensional analysis without relying on a single imaging method.A wide range of conventional and advanced modes-including transmitted widefield,fluorescence widefield,multi-angle oblique illumination,multi-angle TIRF widefield,confocal,and super-resolution imaging-are all supported within one system.

This multi-mode integration offers flexible control over imaging depth and spatial resolution while allowing the same sample to be examined under consistent conditions.As a result,both microscopic and ultra-microscopic structures can be analyzed comprehensively to meet diverse experimental requirements.

90μm×90μm field of view at 100×

At 100×magnification,the NSR1000 delivers a field of view of up to 90μm×90μm,allowing researchers to examine relatively large sample areas at the microscopic level while preserving very high resolution.This wide coverage provides a clear view of both overall structures and fine details,significantly improving observation efficiency and analytical capability.

Twofold improvement in XY resolution with 2D-SIM

Using SIM super-resolution technology,the NSR1000 doubles lateral(XY)resolution.When combined with a 100×high-NA apochromatic objective(NA 1.49),spatial resolution can reach 86 nm,enabling fine subcellular structures-such as mitochondrial cristae-to be clearly resolved and analyzed with greater precision.

Enhanced axial resolution with 3D-SIM

Through a three-beam phase-shifted structured illumination approach,the system achieves tangential axial sectioning,improving Z-axis resolution to approximately 269 nm.This allows the NSR1000 to accurately visualize three-dimensional structures within biological specimens,supporting detailed study of features located deep inside the sample.

Real-time super-resolution imaging

GSIM provides an efficient reconstruction method for super-resolution imaging.During real-time reconstruction,interleaved processing can be used to increase temporal resolution and imaging efficiency,enabling high-speed dynamic processes to be observed at high resolution as they occur.This interleaved mode can also be applied to selected regions within acquired data sets,improving data utilization and enhancing both 2D and 3D super-resolution time resolution.

High-speed system control

The microscope offers fast,responsive digital control over a wide range of motorized components,including objective turret switching,focusing,condenser selection,and fluorescence module changes,allowing users to operate the system with high precision and efficiency.

Multidimensional imaging and visualization

Custom imaging profiles can be stored,and scanning across X,Y,Z,λ,and T dimensions is supported.The system includes multiple acquisition modes such as multi-channel fluorescence imaging,time-lapse capture,multi-position recording,Z-stack imaging,and panoramic stitching.These modes can be freely combined to accommodate complex and diverse experimental workflows.

Deconvolution

Two-dimensional images can be sharpened using deconvolution,with multiple processing cycles available to reduce shot noise in confocal data.Three-dimensional deconvolution is also supported for multidimensional image sets.

Live cell imaging system

For live cell experiments,the system precisely controls stage temperature,humidity,and CO₂levels to create a stable culture environment suitable for extended observations.

Adaptive Focus System(AFM)

The NIB1000 employs an independent focusing architecture that isolates the Z-axis from other mechanical movements.Its Adaptive Focus System automatically compensates for focus drift,maintaining clear and stable images whether using high-NA objectives or advanced techniques such as super-resolution,confocal,or TIRF imaging.

High-performance LED fluorescence illumination

The LED4 system delivers up to four independent LED excitation channels with high energy efficiency and strong output,supporting routine fluorescence imaging with stable brightness.It is designed for continuous operation with a long service life and does not require lamp replacement,helping to minimize photobleaching and phototoxicity for sensitive live samples.

Interactive control interface

The NIB1000 features a front-mounted touch panel that enhances usability and system expandability,while traditional control knobs and buttons are maintained on both sides for intuitive manual operation.This hybrid design allows convenient use even in low-light laboratory environments,enabling researchers to concentrate on their experiments without complex adjustments.

The integrated 5.6-inch touchscreen provides direct access to motorized functions such as objective switching,single-or dual-layer fluorescence filter turret selection,condenser illumination,motorized stage and Z-axis speed,beam splitter control,ESC output,function keys,and objective focusing.It also displays real-time information including objective magnification,illumination level,active fluorescence channel,output port,and XYZ position and movement speed.

Wide-aperture optics and fluorescence illumination

A large-aperture tube lens increases light collection efficiency,and when paired with a wide-field CMOS sensor,enables brightfield and fluorescence imaging across a full 25 mm field of view.The fluorescence illumination unit is optimized for FOV 25 mm and uses high-power LEDs to deliver a broad,high-transmission excitation spectrum-including ultraviolet wavelengths-while large-diameter fluorescence filters provide high signal-to-noise images across the entire field.