Thermodynamic and Kinetic Properties Evaluation

Thermodynamic and kinetic properties evaluation is a suite of advanced analytical techniques designed to characterize the stability, binding behavior, and structural dynamics of biomolecules, including proteins, antibodies, and nucleic acids. These analyses provide critical insights into the energy landscapes (thermodynamics) and time-dependent interactions (kinetics) that govern molecular behavior under physiological or stress conditions. In biopharmaceutical development, this evaluation ensures drug candidates exhibit optimal stability, target engagement, and manufacturability, aligning with regulatory requirements for safety, efficacy, and quality.

Illustration of our four types of thermodynamic and kinetic properties evaluation and their significance. (STEMart Oringinal) Fig 1. Our four types of thermodynamic and kinetic properties evaluation and their significance. (STEMart Oringinal)

Our Services

Protein Melting Temperature (T_m) Analysis

T_m quantifies the thermal stability of a protein by identifying the temperature at which 50% of its structure unfolds. Using techniques like Differential Scanning Calorimetry (DSC) and Fluorescence Thermal Shift Assay (FTSA), we determine T_m to optimize formulations, predict shelf-life, and validate higher-order structures.

Ligand Binding Affinity Evaluation

This service measures the strength of biomolecular interactions via equilibrium dissociation constants K_D. Technologies such as Surface Plasmon Resonance (SPR) and Isothermal Titration Calorimetry (ITC) enable precise affinity ranking for drug candidates, biosimilar comparability, and safety profiling.

Binding Kinetics Analysis

Binding kinetics evaluates the temporal dynamics of molecular interactions, including association Kon and dissociation Koff rates. Using SPR, BLI, GCI and other techniques, we profile target residence time, mechanism of action, and off-target risks for antibodies, small molecules, and gene therapies.

Folding and Unfolding Kinetics Analysis

This analysis examines the rates and pathways of protein structural transitions under denaturing conditions. Techniques like stopped-flow fluorescence and circular dichroism (CD) provide insights into folding mechanisms, aggregation propensity, and excipient effects, guiding stable biologic design.

Why Choose STEMart?

Reasons for choosing STEMart. (STEMart Oringinal)

Cutting-Edge Technology

Label-Free Platforms: SPR, GCI, and MST for high-resolution kinetics.
High-Throughput Solutions: BLI, FP, and AlphaScreen for rapid screening.
Live-Cell Analytics: NanoBRET and TR-FRET for physiologically relevant data.

End-to-End Expertise

Customized protocols for complex targets (membrane proteins, ADCs, AAVs).
Advanced data analysis (global fitting, nonlinear regression).
Regulatory-ready reports compliant with ICH, USP, and FDA standards.

Proven Industry Impact

Accelerated development of 50+ biologics, including mAbs, ADCs, and gene therapies.
Trusted by top 20 pharma companies for critical stability and binding studies.

Thermodynamic and kinetic properties evaluation is the cornerstone of rational drug design, bridging molecular behavior with therapeutic performance. STEMart combines state-of-the-art technologies, regulatory expertise, and customized workflows to deliver actionable insights, from early discovery to commercial quality control.
Contact us to transform your biopharmaceutical development with precision thermodynamics and kinetics.