Cellulose Acetate Electrophoresis

Cellulose Acetate Electrophoresis is one of the zone electrophoresis, and the supporting medium of it is cellulose acetate film. Cellulose acetate is derived from the acetylation of the cellulose hydroxyl group. Under an electric field, the sample migrates on the cellulose acetate film in the buffer system based on the charge.

Fig.1 Illustration of cellulose acetate electrophoresis

• Preparation
  Prepare electrophoresis buffer with a certain pH. Soak suitable-sized cellulose acetate films in the buffer solution for some time. Then use filter paper to absorb the excess buffer on the surface.
• Sample Application
  Apply a certain amount of sample in a strip on the cellulose film with slides or capillary tubes.
• Electrophoresis
  Add an appropriate amount of buffer to the electrophoresis tank to soak the platinum electrode. Cut wicks from filter paper or chromatography paper and place them along the bridges in the tank. Place cellulose films on filter paper wicks, the sample strips near the cathode. Switch on the electrophoresis apparatus and set the voltage and current. Perform electrophoresis for a period of time (45 minutes to 1 hour)
• Determination
  Stain the film and bleach it until the background is colorless. Blow dry the film and cut the separated strips. Put the strips in the liquid elution in test tubes to treat them. Finally, measure the absorbance of each tube with a spectrophotometer.

• Good resolution
• Less time consumption
• Sharp bands without tailing of hydrophilic materials and proteins
• Various thickness layers available for different particle sizes
• Cellulose acetate available to be made transparent for downstream analysis
• Translucent strips suitable for a direct photoelectric scanning
• Low content of glucose suitable for carbohydrate electrophoresis

• An alternative and modification to paper electrophoresis
• Separation and analysis of serum protein, hemoglobin, globulin, lipoprotein, glycoprotein, alpha-fetoprotein, steroid hormones and isoenzymes
• Combination with immuno-electrophoresis and immuno-diffusion

Electrophoresis Systems

An electrophoresis system applies an electric charge to molecules, causing them to migrate toward their oppositely charged electrode. It’s the device for electrophoresis performing and is usually divided into horizontal type and suspension type. The choice of electrophoresis systems depends on the molecule of study, the method of separation and the downstream application.

Electrophoresis Accessories

We provide comprehensive electrophoresis accessories including electrophoresis power supplies, combs, UV blocking mats, exposure cassettes, gel cutting tools, short plates, spacer plates, and so on, for gel preparation and electrophoresis performance in electrophoresis experiments.

pH Meters

A pH meter is an instrument used to determine the pH value of a solution, utilizing the relationship between the electromotive force of the galvanic cell and the hydrogen ion concentration. A pH meter is used to determine the pH value of buffers in electrophoresis experiments. The temperature and ionic strength should be considered in pH measurement.

Slides

Microscope slides are made of glass or quartz and are typically used to place cells or tissue sections to make it convenient when observing things under a microscope. In cellulose acetate electrophoresis experiments, slides can be used to apply a certain amount of sample in a strip on the cellulose film.

Staining Appliances

We provide Gel Staining Boxes made from translucent polypropylene or clear PMP with snap-fit lids. Drain located flush with the bottom minimizes disturbance of the gel during liquid drainage. The box can be used to stain, fix, destain and manipulate gels and membranes conveniently in electrophoresis experiments.

Water Baths

A water bath is laboratory equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time. Most water baths have a digital or an analogue interface to allow users to set a desired temperature.

Spectrophotometers

A spectrophotometer produces a specific wavelength light source through a series of spectrophotometric devices. After the light source passes through the tested sample and is absorbed, the absorption value of the sample is calculated and converted into the concentration of the sample. A spectrophotometer is often used to quantify nucleic acid and protein in electrophoresis experiments.

STEMart provides you with a variety of electrophoresis equipment or consumables to meet your various R&D and application needs. If you have any questions or requirements for cellulose acetate electrophoresis, please feel free to contact us.