Protein Structural Visualization

Understanding the 3D structure of proteins is crucial in pharmaceutical development, as protein conformation directly influences function, stability, and drug interactions. Protein structural visualization provides critical insights into molecular mechanisms, enabling precise drug design, quality control, and formulation optimization. Our Cryo-Electron Microscopy (Cryo-EM) service delivers high-resolution structural data, essential for advancing therapeutic proteins, monoclonal antibodies, and other biologics.

Our Service Based on Cryo-EM

Cryo-EM is a revolutionary imaging technique that allows near-atomic resolution visualization of protein structures without the need for crystallization. By flash-freezing protein samples in vitreous ice and imaging them under an electron microscope, Cryo-EM preserves native conformations and provides detailed structural insights.

Principle:

• Protein samples are rapidly frozen in a thin layer of amorphous ice, preventing ice crystal formation and preserving their native state.
• An electron beam passes through the sample, generating high-resolution images without causing significant radiation damage.
• Computational algorithms reconstruct 3D models, revealing intricate molecular structures at near-atomic detail.

Our Workflow:

Fig.1 Typical workflow of single particle cryo-EM for structural analysis.¹

1. Sample Preparation: Optimization of protein concentration, buffer conditions, and vitrification for high-quality imaging.
2. Cryo-EM Grids Setup: Applying protein samples onto grids, followed by rapid freezing in liquid ethane to preserve native structures.
3. Cryo-EM Imaging: Capturing high-resolution images of vitrified samples using state-of-the-art electron microscopes.
4. Data Collection: High-throughput imaging to acquire thousands of projection images for 3D reconstruction.
5. Data Pre-Processing: Filtering, alignment, and motion correction to enhance image quality and remove artifacts.
6. Map Reconstruction: Computationally assembling 3D electron density maps from 2D image projections.
7. Model Building: Constructing atomic-resolution models based on electron density maps.
8. Structural Analysis: Interpreting and validating the final structure, generating detailed reports with actionable insights.

Advantages of Cryo-EM:

1. High Resolution: Achieves near-atomic resolution, even for large macromolecular complexes.
2. Minimal Sample Requirement: Requires less protein material compared to NMR and crystallography.
3. Preserves Structural Integrity: Analyzes proteins in physiological conditions, capturing dynamic conformational states.

Why Choose Us?

• Cutting-Edge Technology: We utilize the latest Cryo-EM instruments with direct electron detectors for superior imaging quality.
• Expert Scientific Team: Our specialists have extensive experience in protein structural analysis and data interpretation.
• Comprehensive Analytical Services: From sample preparation to final reporting, we provide a complete end-to-end solution.
• Regulatory Compliance: Our services align with industry standards for biopharmaceutical characterization and regulatory submissions.

For more information on our cryo-EM-based Protein Structural Visualization service or to discuss your specific requirements, please contact us today.