Ion-Exchange Chromatography Technology

Ion-Exchange Chromatography Technology is a chromatographic method in which ion-exchange resin or chemically bonded ion exchanger is used as the stationary phase and the difference of ion exchangeability or total charge of the separated components is used to achieve separation. This technique enables the separation of similar types of molecules that would be difficult to separate by other techniques because the charge carried by the molecule of interest can be readily manipulated by changing buffer pH.

Fig.1 Schematic diagram of ion-exchange chromatography principle.

• Packing of chromatographic columns
  The ion exchange separation process is usually carried out in chromatographic columns packed with an ion exchanger.
• Loading of the sample
  After the column is packed, the solution to be assayed is placed on top of the column and allowed to pass through the bed of the ion exchanger with the help of a syringe or pipette.
• Developments of the chromatogram
  Chromatograms were developed with the aid of different mobile phases.
• Analysis of detection of the eluate
  Collecting the different components of the eluate and analyzing its content.
• Regeneration
  After separation, it should not be disposed of due to its cost.

• Fast and convenient: a sample can get the results of 4-7 common ions in about 25 minutes.
• Good selectivity: micrograms per liter - milligrams per liter.
• Column stability: allows the determination of a variety of ionic compounds.

• Living environment: analysis of environmental water, drinking water, domestic sewage, industrial wastewater, etc.
• Biochemical research: separation and purification of inorganic ions, organic acids, nucleotides, amino acids, antibiotics, and other small molecular substances and biological macromolecular substances such as proteins.
• Others: analysis of organic compounds, drugs, pesticides, and surfactants.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

Chromatography Syringes

Syringes and syringe accessories for use with autosamplers, injectors, mass spectrometers, and other chromatography systems. We understand that choosing the right chromatography syringes can be critical to getting accurate results. That’s why you’ll find a large selection of chromatography syringes, including reusable glass, all-polypropylene, 3-part disposable syringes and more.

Pipettes and Pipette Tips

Pipette is a measuring tool that moves liquid from the original container to another container within a certain range. It is widely used in clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, and food laboratories. We provide a variety of pipettes for you to choose to use in a variety of experimental research.

Chromatography Refrigerator

A chromatography refrigerator is a type of refrigerator that is specially designed for use in chromatography. The refrigerators provide temperature uniformity and stability and are available in a variety of models and sizes. Chromatography refrigerators store and protect a variety of materials during the demanding requirements of daily use in research, clinical, and industrial laboratories.

STEMart provides you with a variety of ion-exchange chromatography technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.