Partition Chromatography Technology

Partition Chromatography Technology is a kind of chromatographic method which separates different components by the difference in their partition properties. A polar solvent immobilized on support is called a stationary phase, and it does not move. Elution with another, less polar solvent, called the mobile phase, is always mobile. Both phases are in liquid form. The components of a mixture are separated if their partition coefficient between the solvents is sufficiently different.

The most commonly used carriers include silica gel, diatomaceous earth (Kresel guhr, celite), and cellulose, among others. Silica gel must be deactivated by impregnation with water or other polar solvents before use. The stationary liquid phase and the mobile liquid phase must be immiscible with each other. Otherwise, the two phases merge into one and no partition can occur. The general rule is that the more soluble the solute particles are in the mobile phase, the smaller the partition coefficient and the faster the elution from the column. From this, it can be seen that the more solute particles dissolved in the stationary phase, the larger the partition coefficient, the slower the downward movement spee.

These are some important types of partition chromatography technology.

Column Chromatography Technology – In this technique, liquid stationary phase is taken on silica gel or alumina cellulose powder.

Thin Layer Chromatography Technology – In this technique, a very thin layer of stationary phase is taken.

Paper Chromatography Technology – In this technique, liquid stationary phase is taken on chromatography paper made up of cellulose which is a polar substance. Water molecules get trapped in it.

Fig.1 Display of types of partition chromatography technology.

• Simple operation and inexpensive separation technology.
• High efficiency.
• Simultaneous separation of organic and inorganic compounds.
• More accurate results.
• Short separation time.

• Analysis of environmental material analysis.
• Detection of drug purity and monitoring of chemical synthesis reactions.
• Separation of steroids, bile acids, and mycotoxins.
• Removal of pesticides, phenols, and pesticides.
• Detection of contaminants in beverages and food.

STEMart provides you with a variety of partition chromatography technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

Chromatography Syringes

Syringes and syringe accessories for use with autosamplers, injectors, mass spectrometers, and other chromatography systems. We understand that choosing the right chromatography syringes can be critical to getting accurate results. That’s why you’ll find a large selection of chromatography syringes, including reusable glass, all-polypropylene, 3-part disposable syringes and more.

Thin-layer plate

We offer an extensive portfolio, which includes: conventional TLC plates for manual use, HPTLC plates for high-performance thin-layer chromatography, MS-grade TLC and HPTLC plates for perfect combination to Mass spectrometry, PLC plates for easy adaptation to preparative scale, and specialty plates for special needs.