Supercritical Fluid Chromatography Technology

Supercritical Fluid Chromatography (SFC) is a chromatographic method using supercritical fluid as the mobile phase. The supercritical fluid chromatography technology device consists of a high-pressure mobile phase delivery system, a chromatographic separation system, and a detection system. The detection system is divided into HPLC type and GC type. The most widely used mobile phase in supercritical fluid chromatography technology is supercritical carbon dioxide. In semi-preparative / preparative SFC, carbon dioxide is vaporized when the sample is brought to normal pressure, so post-treatment of the solvent after separation is easy. It is used for the analysis and purification of low to moderate molecular weight, thermally labile molecules and can also be used for the separation of chiral compounds.

Fig.1 Conceptual diagram of supercritical fluid states.

• Sample introduction
  The sample is first introduced into the chromatograph.
  The sample is moved down the column with the mobile phase.
• Sample detection
  The separated components are detected by a detector at the outlet of the column, and a chromatogram is formed by data analysis.
• Fraction collection
  The fraction collection is convenient because the primary mobile phase evaporates leaving only the analyte and a small volume of polar co-solvent.

• High column efficiency: the column efficiency of the SFC method is generally higher than that of the HPLC method. Due to the low viscosity of the fluid, the flow rate is faster than that of the HPLC method, which is beneficial to shorten the separation time.
• Selection competition: the role of the mobile phase in SFC is similar to that of the mobile phase in LC. The fluid used as the mobile phase not only carries the solute to move, but also produces an interaction force with the solute.
• Suitable for low temperature: the partial pressure of macromolecular substances is very large, and the temperature much lower than that of GC can be applied to achieve effective separation of macromolecular substances, thermally unstable compounds, high polymers, etc.

• Drug research and development: separation and analysis of new compounds, chiral compounds and the preparation of standards.
• Food: rapid screening of multiple pesticide residues in food, and research on chiral pesticides in food and the environment.
• Agriculture: separation and analysis of pesticides.
• Others: separation and analysis of substances such as surfactants, polymers, polymers, explosives and rocket propellants.

Autosamplers and Injectors

Devices for automated sample injection on gas, ion, liquid (LC), and HPLC columns; includes accessories and replacement parts. Choose from a wide range of high-precision, low-cost, metal-free, high-sample-capacity autosamplers and manual injectors to suit your application needs.

Chromatography Pumps

Chromatography pump is an associated equipment of overall chromatography system. These pumps are used to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material, leading to the separation of the sample components. We offer a variety of chromatography pumps for your choice.

Chromatography Systems

Chromatography systems include all the equipment needed for sample separation, a power source and program software. Important considerations when selecting a chromatography system are the chemical and physical properties of the substance such as chemical reactivity, size, mass and shape and requirements of the laboratory, such as flow process, validation requirements and sample tracking.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Detector

A chromatography detector is a device used in gas chromatography (GC) or liquid chromatography (LC) to detect components of the mixture being eluted off the chromatography column. The function of the detector is to convert the change of the sample composition and content in the chromatographic column effluent into a signal that can be detected.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

STEMart provides you with a variety of supercritical fluid chromatography Technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.