Adsorption Chromatography Technology

Adsorption Chromatography Technology separates different components by using the difference in their physical adsorption properties on the surface of the adsorbent. Adsorption Chromatography Technology is also known as Liquid-Solid Chromatography Technology. The mobile phase is a liquid, and the stationary phase is an adsorbent with less active chemical properties and a large surface area (such as activated carbon, silica gel, etc.). Adsorption Chromatography Technology can be carried out with the adsorbent packed in a column, covered on a plate, or impregnated in porous filter paper, suitable for separating different kinds of compounds (separating alcohols and aromatic hydrocarbons).

A substance that is generally porous in nature with a high surface area to adsorb substances on its surface by intermolecular forces is called an adsorbent. Some commonly used adsorbents are silica gel, alumina, activated carbon, and polyamide.

• Silica gel: suitable for chromatographic separation of neutral or acidic components.
• Alumina:
  Acidic alumina (pH4~5): suitable for separating acidic compounds.
  Basic alumina (pH9~10): suitable for separating basic compounds.
  Neutral alumina (pH7): suitable for separating alkaloids, volatile oils, terpenes, steroids, glycosides, esters, and other compounds that are unstable in acid and alkali.
• Activated carbon: suitable for simple adsorption processes in operations such as decolorization and deodorization in the purification process.
• Polyamide: suitable for phenols, flavonoids, quinones, and fatty carboxylic acids.

These are some important types of adsorption chromatography technology.

Column Chromatography Technology – In this technique, the adsorbent is placed in a column.

Thin-Layer Chromatography Technology – In this technique, the adsorbent is placed on a thin-layer plate.

Paper Chromatography Technology – In this technique, paper is used as the adsorbent.

Fig.1 Display of types of adsorption chromatography technology.

Adsorption Chromatography Technology is widely used in the field of biotechnology, mainly in the separation of small biological molecules. Small biological molecules have small relative molecular mass, relatively stable structure and properties, and less demanding operating conditions.

• Isolation of amino acids.
• Isolation of antibiotics.
• Identification of carbohydrates.
• Separation and identification of fats and fatty acids.
• Isolation and identification of peptides and proteins.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

Thin Layer Chromatography

Thin layer chromatography is performed on a sheet or plate (about 0.25mm thick) of glass, aluminum foil or plastic which is coated with a thin layer of solid adsorbent material. We have a large selection of TLC related products to meet your experimental needs.

Pipettes and Pipette Tips

Pipette is a measuring tool that moves liquid from the original container to another container within a certain range. It is widely used in clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, and food laboratories. We provide a variety of pipettes for you to choose to use in a variety of experimental research.

Chromatography Syringes

Syringes and syringe accessories for use with autosamplers, injectors, mass spectrometers, and other chromatography systems. We understand that choosing the right chromatography syringes can be critical to getting accurate results. That’s why you’ll find a large selection of chromatography syringes, including reusable glass, all-polypropylene, 3-part disposable syringes and more.

Thin-layer plate

We offer an extensive portfolio, which includes: conventional TLC plates for manual use, HPTLC plates for high-performance thin-layer chromatography, MS-grade TLC and HPTLC plates for perfect combination to Mass spectrometry, PLC plates for easy adaptation to preparative scale, and specialty plates for special needs.

TLC Spray Box

The reagent/chemical spraying on the TLC plates are generally carried out inside a fume hood or commercially available TLC spray cabinet with blower and exhaust hose. The TLC plate can be placed supported upright or on a paper inside the spray box. Spraying the box ensures complete removal of reagent mist.

STEMart provides you with a variety of adsorption chromatography technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.