Detection of Vitamins in Plant Tissues

Plant-derived vitamins are a kind of micronutrient which is not only required for the regulation of cell growth, development and metabolism of plant, but also essential to maintain human health. Vitamins are a large family, dozens of vitamins are known at present, which can be roughly classified into fat-soluble and water-soluble categories depending on their hydrophobicity.

The detection of plant vitamins generally includes two steps: extraction and purification of vitamin, and identification of vitamin.

STEMart is able to efficiently and accurately detect the change of vitamins in plant samples with high-performance liquid chromatography, liquid chromatography-mass spectrometry, and biochemical methods.

Methods for Vitamin Extraction and Purification

• Ultrasonic assisted extraction (UAE)
• Supercritical fluid extraction (SFE)
• Solid phase extraction (SPE)
• Liquid–liquid extraction (LLE)
• Dispersive liquid–liquid micro-extraction (DLLME)

Methods for Vitamin Identification

• Chromatography methods
• Electrophoretic methods
• Microbiological assays
• Enzyme-linked immunosorbent assay (ELISA)
• Biosensors
• Fluorescence spectrometry

Test Capabilities

• Detection of water-soluble vitamins
  • Vitamin B1 (VB1) / thiamine detection
  • Vitamin B2 (VB2) / riboflavin detection
  • Vitamin B5 (VB5) / pantothenic acid detection
  • Vitamin B6 (VB6) / pyridoxine detection
  • Vitamin H (VH) / vitamin B7 (VB7) / biotin detection
  • Vitamin B9 (VB9) / folic acid detection
  • Vitamin B12 (VB12) detection
  • Vitamin B17 detection
  • Vitamin C (VC) / ascorbic acid (AA) detection
• Detection of fat-soluble vitamins
  • Vitamin A (VA) detection
  • Detection of vitamin D3 (VD3) / cholecalcidol
  • Vitamin E (VE) / tocopherol detection
  • Determination of vitamin K1 (VK1) / chlorophyll quinone

For more information about our plant vitamins detection service, please contact us.