Surface Plasmon Resonance (SPR)

Surface Plasmon Resonance (SPR) is a powerful optical phenomenon that occurs when polarized light, under total internal reflection, excites free electrons at the surface of a thin metal film. Any change in the refractive index near the metal surface alters the resonance angle (SPR angle) of the incident light. By continuously recording these changes, detectors can capture the dynamic process of biomolecular interactions in real time within microfluidic channels attached to the chip surface.

Applications

Protein–Protein Interaction Studies

Small and Large Molecule Drug Discovery

Antibody Screening and Characterization

Process Development and Quality Control

SPR Applications (STEMart Original)

SPR Advantages (STEMart Original)

Advantages

Activity Analysis: Directly measure biomolecular function and interaction strength

Real-Time: Monitor binding events as they occur

Label-Free: No need for fluorescent or radioactive labeling

High Sensitivity: Detect even low-affinity and low-concentration interactions

High Resolution: Resolve fine kinetic parameters with precision

Reliable Data: Reproducible results trusted in research and industry

Understanding the SPR Sensorgram

Illustration of the SPR Sensorgram (STEMart Authorized)

Phase	Meaning
Baseline	The initial signal when buffer flows across the sensor surface before testing begins.
Association	The association process as analytes bind to immobilized ligands.
Equilibrium	The point where binding and dissociation reach dynamic balance.
Dissociation	The process of buffer washing away bound analytes.
Regeneration	Flushing the chip surface to remove remaining analytes while preserving ligand activity for subsequent runs.

Our SPR Product Families

Polariton Series (SPR)

Advanced molecular interaction analysis systems (S-CLASS, P-CLASS, R-CLASS) designed for high sensitivity, high throughput, and flexible workflows in life science and industrial applications.

WeSPR Series (MetaSPR)

Next-generation platforms (WeSPR One, One Auto, 100X, 200, HT8, Imager, Cell Analyzer SP) based on Meta-Surface Plasmon Resonance (MetaSPR) nanostructured chip technology, offering higher sensitivity, broader sample compatibility, and reduced operating costs.

Whether you are advancing basic research, driving drug discovery, or ensuring biopharmaceutical quality control, our Polariton Series and WeSPR Series deliver the sensitivity, flexibility, and throughput required.