Immunoelectrophoresis

Immunoelectrophoresis combines AGAR electrophoresis and two-way AGAR diffusion with the antigen-antibody reaction to separate and characterize proteins. When an electric field is applied to an agarose gel slide, a mixture of antigens present in the wells is separated into individual antigens based on size and charge. Antisera in the groove migrate towards antigens and react with them, forming separate precipitation arcs.

Immunoelectrophoresis can continue to be classified as one-dimensional immunoelectrophoresis, two-dimensional quantitative immunoelectrophoresis, rocket-immunoelectrophoresis, and fused rocket immunoelectrophoresis.

Fig.1 Illustration of immunoelectrophoresis

• Preparation
  Prepare the agarose gel glass slide. Dilute antigens and antibodies.
• Sample Application
  Add the antigen solution to the small well in the agarose gel slide.
• Electrophoresis
  Switch on the electrophoresis apparatus and set the voltage and current. Perform electrophoresis for a period of time.
• Antigen-antibody reaction
  Dug a horizontal groove in the agarose gel slide and add the antisera solution to it to make antigens and antibodies spread and react.
• Determination
  Stain, decolorize, and dry the gel slide. Separated precipitation arcs are formed where the ratio of antigen to antibody is optimal. Analyze the composition of the sample according to the number, location, and shape of precipitation arcs, referring to the electrophoretic diagram formed by known antigens and antibodies.

• High specificity of antigen-antibody reaction
• High sensitivity and great resolution
• Less time consumption
• Lower sample volume required

• Protein identification in immunology
• Qualitative and quantitative analysis of proteins in serum
• Identification of single antigen in antigen's mixture
• Monitoring purity of antigen-antibody and antigen
• Diagnosis of multiple myeloma, hypogammaglobulinemia, polyclonal and monoclonal gammopathies, parvovirus infection, encephalitis, meningococcal meningitis, Haemophilus influenzae infection, etc.

Electrophoresis Systems

An electrophoresis system applies an electric charge to molecules, causing them to migrate toward their oppositely charged electrode. It’s the device for electrophoresis performing and is usually divided into horizontal type and suspension type. The choice of electrophoresis systems depends on the molecule of study, the method of separation and the downstream application.

Electrophoresis Accessories

We provide comprehensive electrophoresis accessories including electrophoresis power supplies, combs, UV blocking mats, exposure cassettes, gel cutting tools, short plates, spacer plates, and so on, for gel preparation and electrophoresis performance in electrophoresis experiments.

Micro Sample Syringes

We provide Micro Sample Syringes with a variety of volumes and high accuracy to help you achieve fast and accurate sampling in electrophoresis experiments.

Pipettes

Pipettes are used to aspirate and dispense larger and specific amounts of liquid. They made be reusable or disposable, made from glass or plastic (typically polystyrene), nonsterile, sterile, or autoclavable, and cotton-plugged or plain. Accurate liquid measurements, dilutions, and transfer is key to most successful laboratory assays and analyses.

Staining Appliances

We provide Gel Staining Boxes made from translucent polypropylene or clear PMP with snap-fit lids. Drain located flush with the bottom minimizes disturbance of the gel during liquid drainage. The box can be used to stain, fix, destain and manipulate gels and membranes conveniently in electrophoresis experiments.

Gel Drying Equipment

We provide gel drying equipment with drying frames, assembly table, cellophane, gel drying solution, and other accessories to dry the microgel between two sheets of cellophane, for the preservation of polyacrylamide and agarose gels. Once dry, the gel is clear with a glossy finish and is ideal for use in density measurements or as a photographic record for long-term storage in a lab notebook.

STEMart provides you with a variety of electrophoresis equipment or consumables to meet your various R&D and application needs. If you have any questions or requirements for immunoelectrophoresis, please feel free to contact us.