Column Chromatography Technology

Column Chromatography Technology is a distribution method with distribution balance as the mechanism. The chromatographic system consists of two phases, one is the stationary phase and the other is the mobile phase. When the two phases move relative to each other, the differences in the distribution equilibrium properties of the components contained in the mixture are used repeatedly, and finally, the purpose of separation is achieved. This technique can be used on a small scale as well as a large scale to purify materials that can be used in future experiments. It is a common method for the purification and separation of organic or inorganic substances.

Fig.1 Schematic diagram of column chromatography technology principle.

• Adsorbent filling
  The adsorbent is filled into the column as the stationary phase. The stationary phase is wetted with the help of solvent. The mobile phase or eluent is either a solvent or a mixture of solvents.
• Sample introduction
  The mixture of compounds to be separated is added from the top of the column without disturbing the top level.
• Sample elution
  The eluent is added from the top of the column, adjusting the appropriate flow rate. When the compound to be separated arrives at the end of the column, it is collected in a clean test tube.

• There is no chemical change with the adsorbed material.
• The components to be separated can be adsorbed reversibly.
• The particle size is appropriate.
• The price is colorless for easy observation.

• This process involves the separation of any mixture.
• Impurities can be separated from any mixture.
• Any variety and quantity can be separated.
• This process is cheap and easy to understand.
• A wide variety of solvents can be used in this process, which will give you the results you need.
• The process can be fully automated by large laboratories.

• It is used to isolate the active ingredients.
• It helps separate complex mixtures.
• It is used to determine drug estimates from drug formulations.
• It is used to remove impurities.
• It is used to separate metabolites from biological fluids.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

Autosamplers and Injectors

Devices for automated sample injection on gas, ion, liquid (LC), and HPLC columns; includes accessories and replacement parts. Choose from a wide range of high-precision, low-cost, metal-free, high-sample-capacity autosamplers and manual injectors to suit your application needs.

Chromatography Refrigerator

A chromatography refrigerator is a type of refrigerator that is specially designed for use in chromatography. The refrigerators provide temperature uniformity and stability and are available in a variety of models and sizes. Chromatography refrigerators store and protect a variety of materials during the demanding requirements of daily use in research, clinical, and industrial laboratories.

STEMart provides you with a variety of column chromatography technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.