NCF1000 Confocal Microscope

Collect more information from every sample

With its advanced 25 mm field of view,the NCF1000 confocal microscope can image large specimens in a single acquisition,delivering up to 1.5×higher data throughput compared with earlier microscope generations.When combined with scanning resolutions of up to 8192×8192 pixels,the system meets the growing need for detailed analysis of tissues,organs,and living or model organisms,providing rich biological data for life science research.

Large,high-resolution images with fewer tiles

When paired with the NIB1000 inverted microscope,the NCF1000 can generate high-quality confocal images across a full 25 mm field of view.The expanded imaging area significantly reduces the number of individual frames needed for image stitching,shortening acquisition time while maintaining uniform brightness,high efficiency,and high-throughput performance for large samples.

High-efficiency scanner and detection system

NIS Series objectives are designed with high numerical aperture,long working distance,and advanced chromatic aberration correction.Their multi-layer optical coatings deliver high image clarity and resolution,making them well suited for both standard optical microscopes and confocal imaging platforms.

The NCF1000 integrates a high-precision galvanometer scanning system and a continuously adjustable electronic pinhole within the scan head to achieve low noise,high contrast,and high-quality confocal imaging.With a maximum scan size of 8192×8192 pixels,even low-magnification objectives can provide fine spatial sampling,ensuring accurate capture and faithful reproduction of microscopic details.

Higher scanning speed

Real-time four-channel synchronous imaging with frame rates up to 60 fps(8×256 pixels)shortens exposure time under high illumination and greatly reduces phototoxic effects.The high-speed acquisition enables continuous,high-frequency data capture to record dynamic events and long-term sample changes in real time,supporting the demanding imaging requirements of life science research.

Real-time four-channel synchronous imaging

With imaging speeds of up to 60 fps(8×256 pixels),the NCF1000 minimizes exposure under high illumination and significantly lowers phototoxic effects.Its high-speed acquisition supports continuous data collection,enabling accurate recording of dynamic processes and long-term changes to meet the demanding needs of life science imaging.

The NCF1000 features advanced four-channel fluorescence fusion technology,allowing multiple fluorescence signals to be captured and combined simultaneously in real time.Four different fluorescent labels can be detected and analyzed within the same field of view,with seamless channel switching and spectral separation,revealing complex multi-dimensional information while improving experimental efficiency and data accuracy.

High-speed hardware control

The system provides intuitive digital management and precise control of multiple motorized components within the microscope,including objective switching,focusing,condenser selection,and fluorescence module changes.

Multidimensional imaging and visualization

User-defined observation modes can be stored,supporting combined scanning across X,Y,Z,λ,and T dimensions.A range of flexible acquisition modes is built in,including multi-channel fluorescence imaging,time-lapse recording,multi-position capture,Z-stack imaging,and panoramic stitching.These modes can be freely combined to suit a wide variety of complex experimental applications.

Deconvolution

Two-dimensional images can be sharpened through deconvolution,with repeated processing available to reduce shot noise in confocal data.Three-dimensional deconvolution is also supported for multidimensional image sets.

High-speed motorized control

Live Cell Imaging System

Designed for live cell observation,the system precisely controls the temperature,humidity,and CO₂levels of the sample stage,creating a stable and suitable environment for long-term experiments.

Adaptive Focus System(AFM)

The NIB1000 features an independent focusing mechanism that minimizes the influence of other mechanical components on the Z-axis.Its Adaptive Focus System automatically corrects focus drift,ensuring sharp and stable images whether using high-NA objectives or advanced techniques such as super-resolution,confocal,or TIRF imaging.

High Specification LED Fluorescent Lighting System

The LED4 light source provides up to four independent illumination channels with high excitation efficiency and strong brightness to support routine fluorescence imaging.It offers long service life with continuous operation,eliminates the need for bulb replacement,and helps reduce photobleaching and phototoxicity for sensitive samples.

Interactive Operation

The front panel of the NIB1000 incorporates a touch screen that enhances usability and system expandability,while traditional control knobs and buttons are retained on both sides for convenient operation.This design allows easy control even in low-light environments,enabling researchers to focus on their experiments without complex adjustments.

The 5.6-inch touch display allows direct control of key motorized components,including objective switching,fluorescence filter selection,condenser light intensity,stage and Z-axis movement,beam splitter settings,and user-defined function keys.It also shows real-time information such as objective magnification,illumination level,active fluorescence channel,output port,and XYZ position and speed.

Wide aperture optical system and fluorescent illumination

The system uses a large-aperture tube lens to significantly increase light throughput,which,when combined with a wide-field CMOS sensor,supports brightfield and fluorescence imaging across a full 25 mm field of view.The fluorescence illumination module is optimized for FOV 25 mm and employs high-power LEDs to deliver a broad,high-transmission excitation spectrum,including ultraviolet wavelengths.Matched large-diameter fluorescence filters ensure high signal-to-noise imaging across the entire field.