Cellulose Acetate Electrophoresis (CAE) Technology

Cellulose acetate electrophoresis (CAE) is a type of zone electrophoresis that separates molecules in liquid form such as proteins on a support matrix made of cellulose acetate under an electric field. As with typical zone electrophoresis, electrophoretic separation occurs in a homogeneous buffer system. The support matrix in the form of sheets or stripes is submerged in an electrophoresis running buffer maintained at a definite pH value, and electrophoretic separation is conducted for a definite time. Cellulose acetate electrophoresis is suitable for rapid screening purposes by comparing the pattern of separation obtained from the sample of interest with that of a known reference. The technique is applicable to various types of samples, such as nucleic acids, proteins, polypeptides dyes, and mixtures of dyes and polysaccharides.