Molecular Hybridization Technology

The nucleic acid hybridization is the process wherein two DNA or RNA single chains (mono-stranded) from different biological sources, make the double catenary configuration, based on contingent sequence homology of the two sources, resulting DNA-DNA, RNA-RNA or DNA-RNA hybrids. Hybridization on a solid carrier is: Southern blotting, Northern blotting, Western blotting. The purpose of the hybridization techniques is identification or localization of certain nucleic acid sequences (genes) in the genome of some species.