Affinity Chromatography Technology

Affinity chromatography is a method for separating biomolecules from a mixture based on highly specific macromolecular binding interactions between a biomolecule and another substance. The specific type of binding interaction depends on the biomolecule of interest; antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid binding interactions are frequently exploited for isolation of various biomolecules.

In a typical affinity chromatography experiment, ligands are attached to a solid, insoluble matrix to form stable covalent bonds. First load the stationary phase into the column that introduces the mobile phase. Molecules bound to the ligand will remain bound to the immobilized phase. A wash buffer is then used to remove non-target biomolecules by disrupting their weaker interactions with the stationary phase, while biomolecules of interest will remain bound. The target biomolecule can then be removed by using a so-called elution buffer, which disrupts the interaction between the bound target biomolecule and the ligand. The target molecule is thus recovered in the eluting solution. Compared with other chromatographic methods, affinity chromatography has higher separation selectivity and separation resolution.

Fig.1 Schematic diagram of affinity chromatography principle.

• Preparation of column
  The column is packed with solid, insoluble carriers, such as sepharose, agarose, cellulose, etc. Ligands attached on the carrier are selected based on the analyte of interest.
• Loading of sample
  The solution containing the mixture is poured into the elution column and run at a controlled rate.
• Elution of complex
  The target substance is recovered by changing conditions to favor the elution of the bound molecules.
• Regeneration
  After separation, it should not be disposed of due to its cost.

• Simple operation, high efficiency, and mild conditions are very beneficial to the purification of unstable proteins.
• The protein is concentrated in the purification process, and when combined with the affinity ligand, the properties are more stable and the activity recovery rate is higher.

• For the separation of living macromolecular materials, viruses, and cells.
• For the separation and purification of nucleic acids and proteins from cell extracts, and for the separation of antibodies from plasma.
• For the study of specific interactions.

Chromatography Columns

Chromatography is an analytical technique that separates components in a mixture. Chromatographic columns are part of the instrumentation that is used in chromatography. Columns play a central role in the performance of chromatographic process. Well-packed columns with consistent performance in the whole design space of a process will deliver consistent product recovery and separation from contaminants.

Chromatography Fraction Collector

The fraction collector is typically a rotating rack that can be filled with test tubes or similar containers. If the chromatographic separation is not for pure chromatographic analysis, but for other spectral identification, or to obtain small-scale preparation of a small number of test samples, fraction collection is necessary, and fraction collector collection is ideal and accurate in operation.

Chromatography Syringes

Syringes and syringe accessories for use with autosamplers, injectors, mass spectrometers, and other chromatography systems. We understand that choosing the right chromatography syringes can be critical to getting accurate results. That’s why you’ll find a large selection of chromatography syringes, including reusable glass, all-polypropylene, 3-part disposable syringes and more.

Pipettes and Pipette Tips

Pipette is a measuring tool that moves liquid from the original container to another container within a certain range. It is widely used in clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, and food laboratories. We provide a variety of pipettes for you to choose to use in a variety of experimental research.

STEMart provides you with a variety of affinity chromatography technology equipment to help you quickly and efficiently separate and analyze to meet your various R&D and application needs. If you have any questions or requirements for chromatography equipment, please feel free to contact us.