Phosphoflow Service Based on Flow Cytometry Systems

STEMart provides advanced Phosphoflow services utilizing state-of-the-art flow cytometry systems. These systems play a critical role in our laboratory, enabling the precise analysis of phosphorylation states within cells, a key indicator of signal transduction and cellular activation. This technology is essential for research in cellular signaling, drug development, and understanding disease mechanisms at a molecular level.

Flow cytometry systems used for Phosphoflow analysis detect phosphorylated proteins within individual cells using specific phospho-antibodies. This allows for the simultaneous measurement of multiple signaling pathways within a heterogeneous cell population. The ability to capture dynamic changes in phosphorylation states makes this technology invaluable in fields such as oncology, immunology, and pharmacology.

Leveraging our advanced flow cytometry systems, we offer a comprehensive suite of Phosphoflow services, tailored to meet the specific needs of your research. These services ensure accurate, reproducible results for downstream analytical applications.

What We Offer

• Phosphoprotein Detection

Accurate detection and quantification of phosphorylated proteins within cells, enabling detailed analysis of signaling pathways.

• Multiplex Phosphoflow Assays

Simultaneous measurement of multiple phosphoproteins, providing a comprehensive overview of cellular signaling events.

• Custom Phospho-Antibody Panels

Development of tailored panels to measure specific phosphorylated targets based on your research focus.

• Quantitative Analysis

Precise quantification of phosphorylation levels, essential for comparing different experimental conditions and treatments.

• Pathway-Specific Analysis

Detailed analysis of specific signaling pathways, crucial for understanding cellular responses to stimuli or therapeutic interventions.

Advantages of Services Using Our Flow Cytometry Systems

• High Sensitivity and Specificity

Our flow cytometry systems offer precise detection of phosphorylated proteins, ensuring accurate analysis of cellular signaling.

• Simultaneous Multiplexing

Ability to analyze multiple signaling pathways in a single experiment, saving time and providing comprehensive data.

• Dynamic Cellular Insights

Capture real-time changes in phosphorylation states, offering valuable insights into the kinetics of cellular signaling.

• Reproducible Results

High-quality instrumentation ensures consistent and reproducible results across multiple experiments and samples.

Our Equipment

BD LSRFortessa

A versatile flow cytometer equipped with multiple lasers and detectors, ideal for detailed phosphoprotein analysis across various cell types.

Cytek Aurora

This spectral analyzer provides high-resolution detection of phosphorylated proteins, allowing for complex, multiparametric analysis.

BD FACSCelesta

A highly sensitive system designed for accurate detection of phosphoproteins, enabling the detailed study of cellular signaling pathways.

Thermo Fisher Attune NxT

Combines high throughput with precision, making it well-suited for large-scale phosphoflow studies in diverse research applications.

STEMart provides high-quality flow cytometry services tailored to meet the specific needs of our clients. Our experienced specialists use advanced equipment and follow strict quality control measures to ensure accurate and reliable results. Contact us to learn more about our flow cytometry services and how we can help you achieve your research goals.