RNA Antisense Purification to Identify proteins complementary to RNA (CAT#: STEM-MB-0167-WXH)

Introduction

RAP technology (RNA antisense purification) biotin-labels oligonucleotides complementary to the target RNA, thereby capturing related proteins. Next-generation sequencing or mass spectrometry can be used to identify proteins that are complementary to RNA, and to clarify the genomic regions where these interactions occur.




Applications

• Study of RNA-RNA and RNA-protein interactions.
• Screening and specifying the downstream target RNA or protein of the target RNA.
• Screening and identifying proteins that regulate RNA, such as RNA methylation modification enzymes, RNA shearing enzymes, etc.

Procedure

1. Probe Design and Synthesis
2. Cross-linking
3. Lysis of Cells and Removal of DNA
4. Probe preparation and hybridization
5. Capture and purification
6. Elution and collection
7. Detection analysis
qPCR, high-throughput sequencing to analyze the RNA bound to the target RNA
WB or mass spectrometry to analyze the protein bound to the target RNA.

Notes

Customer provides cell or tissue samples