MS2-RIP to study IncRNA-miRNA interaction (CAT#: STEM-MB-0162-WXH)

Name: MS2-RIP to study IncRNA-miRNA interaction
SKU: STEM-MB-0162-WXH
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Introduction

MS2-RIP technology mainly uses Protein G magnetic beads against GFP antibody to precipitate the corresponding RNA-protein complex, and then after separation and purification, q-PCR verification or sequencing analysis can be performed on the RNA bound to the complex.

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Applications Procedure

Applications

The research of interaction between lncRNA and miRNA.

Procedure

1. Vector construction and transfection
2. Acquisition of cell lysate and preparation of magnetic beads
3. Immunoprecipitation of RNA-binding proteins
4. RNA purification
5. RNA reverse transcription and cDNA verification
6. Data analysis

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