HuProt™ 20K Human Proteome Chip to monitor protein–protein interaction (CAT#: STEM-MB-0151-WXH)

Introduction

The protein chip is a high-throughput monitoring system that monitors the interaction between protein molecules through the interaction between target molecules and capture molecules.
The HuProt™ 20K human proteome chip is the highest-throughput protein chip in the world so far. There are 19,394 full-length human proteins on the chip, covering 75% of the ORF region of the human genome. Recombinant proteins are expressed and purified one by one using yeast expression system. Each protein on the chip is equipped with technical replicates and multiple quality control points to ensure that the experimental system is stable and reliable.

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Applications Procedure Notes

Applications

• Protein-protein interactions: tumor invasion, microbial infection, immunity, drug resistance, signaling network.
• Small molecule-protein interaction: drug target screening, signal regulation, cell function regulation.
• Nucleic acid-protein interaction: gene repair, transcriptional regulation, RNA toxicity, lncRNA regulation mechanism.
• Lipid-protein interaction: microbial infection, tumor invasion, drug resistance, regulation of lipid metabolism.
• Screening of autoantibody spectrum: development of disease markers (Biomarker), research on disease mechanism.
• Enzyme substrate screening: post-translational modification regulation, signaling network, protein function.
• Antibody specificity detection: antibody specificity evaluation, antigen identification, vaccine development.

Procedure

1. Protein extraction
2. Sample Incubation
3. Biotin labeling
4. Chip hybridization, washing, staining, scanning
5. Data Analysis

Notes

Customers provide cell or tissue samples.

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