DNA Pull-down to study DNA-protein interactions (CAT#: STEM-MB-0165-WXH)

Introduction

DNA pull-down centers on a DNA sequence of interest and searches for proteins that bind to the sequence. The most common application is to find transcription factors in the promoter region of a specific gene. The principle is: biotin-labeled DNA fragments are bound to streptavidin magnetic beads, and then incubated with nucleoproteins to capture and purify proteins that interact with DNA fragments. On the one hand, the captured protein can be verified by Western blot whether a specific protein binds to the target DNA fragment; on the other hand, it can also be identified by mass spectrometry to screen out proteins that may interact with the DNA fragment.