RNA Pull-down to study RNA-protein interactions (CAT#: STEM-MB-0166-WXH)

Introduction

RNA pull-down, as the core technology for studying the interaction between RNA and protein, is to use in vitro transcription method to label biotin RNA probe, and then incubate with cytoplasmic protein extract to form RNA-protein complex. The complex can be combined with streptavidin-labeled magnetic beads, and through the non-covalent affinity between biotin and streptavidin, it can be separated from other components in the incubation solution. After the complex is eluted, if the detected protein is a known protein, Western blot can be used to detect whether the specific RNA-binding protein interacts with RNA; if an unknown protein is detected, the specific RNA-binding protein can be identified by combining mass spectrometry (MS) to Identify protein types.