Electrophoretic Mobility Shift Assay (EMSA) to detect protein-nucleic acid interaction (CAT#: STEM-MB-0156-WXH)

Introduction

Electrophoretic mobility shift assay (EMSA), is a technique used to detect the interaction between protein and nucleic acid.
Principle: Design specific and non-specific probes according to the experiment. When the nucleic acid probe is mixed with the protein sample for incubation, the protein in the sample that can bind to the nucleic acid probe forms a protein-probe complex with the probe. Due to the large molecular weight of this complex, it migrates slowly during PAGE electrophoresis, while the probe without binding protein migrates faster. After the incubated samples are subjected to PAGE electrophoresis and transferred to the membrane, the protein-probe complex will form a band at the front of the membrane, indicating that there is a protein interacting with the target probe.
Advantages: It can detect a wide range of nucleic acid molecules and proteins, is simple and easy to perform, and can provide accurate positions of protein binding on nucleic acids