Bimolecular fluorescence complementation(BiFC) to study protein-protein interactions (CAT#: STEM-MB-0161-WXH)

Introduction

Bimolecular fluorescence complementation (BiFC) is a technology that can quickly and intuitively determine the location and interaction of target proteins in living cells. The principle of this technology is to subtly divide the fluorescent protein into two non-fluorescent molecular fragments (N-fragment, C-fragment), and fuse the two fluorescent protein fragments with two target proteins A and B respectively. If Proteins A and B interact with each other, and when they are close to each other in space, they will be rebuilt into a complete active fluorescent protein molecule. Under the excitation of the excitation light, the fluorescent protein emits fluorescence. Conversely, if there is no interaction between protein A and protein B, it cannot be excited to fluoresce.