Surface Plasmon Resonance (SPR) to detect biomolecular interactions (CAT#: STEM-MB-0158-WXH)

Introduction

Surface plasmon resonance technology (SPR) is a label-free detection method developed in the 1990s, based on SPR detection biosensor chip to detect the interaction between biomolecules.
SPR is a biosensing analysis technology that uses traditional optical phenomena to generate evanescent waves in different media and resonate with plasmonic waves, and then build biomolecular interactions to detect the interaction between the ligand and the analyte on the biosensing chip.

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Applications Procedure

Applications

• Detection of antigen-antibody, DNA-protein, and DNA-DNA interactions.
• Evaluation of Immunogenicity.
• Determination of binding activity, evaluation of potency of active ingredients of drugs.
• Detection of host cell DNA residues.
• Control of Microbial Contamination.

Procedure

1.Express and purify ligands and analytes
2.Choose appropriate sensor chip
3.Determining the pH and ligand concentration for optimal immobilization
4.Regeneration scouting and surface performance test
(Regeneration is the process of removing bound analyte from the sensor chip after sample injection, without affecting the activity of the immobilized ligand.)
5.Investigate protein binding.
6.Run kinetic assay accompany with binding experiment.
7.Data analysis and kinetic constants determine.

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