Affinity Purification Mass Spectrometry(AP-MS) (CAT#: STEM-MB-0155-WXH)

Introduction

Similar to conventional Co-IP experiments, except that the affinity purification method does not need to introduce antibodies. The tag is fused and expressed with a specific gene, the fusion protein is affinity-purified with streptavidin (strep) magnetic beads, and eluted with biotin to obtain a protein complex. The eluted proteins in the experimental group and the control group were subjected to mass spectrometry, and the proteins in the control group were deducted from the results of the experimental group to identify the proteins that interacted with the target protein.
Technical advantages: The identified interacting protein interacts naturally with the bait protein in vivo, which is closer to the physiological state in vivo; after only one step of affinity purification, proteins with weak interactions with the bait protein may also be identified.