Biolayer Interferometry(BLI) to detect biomolecular interactions (CAT#: STEM-MB-0157-WXH)

• Determination of various biological systems.
• Real-time analysis and detection of interactions between biomolecules.
• Accurate quantification of biomolecules and real-time.
• Label-free analysis of biomolecular dynamics and affinity.
• Observation of the assembly process of protein complexes.
• Screening of molecular markers in tumors.
• Nucleic acid-protein interaction analysis.
• Detection of viral particle-protein, protein-protein interactions.
• Titer identification of a certain trace protein in the vaccine and vaccine development.
• Affinity and kinetic assays for antibodies and small molecule drugs.
• Screening of small molecule compounds (antibodies), etc.

1. Immerse the biosensor in the buffer for equilibration.
2. Solidification: the biotinylated antigen in the solution binds to the surface of the biosensor to increase the thickness of the surface film layer.
3. Immerse the sensor with a known concentration of antigen immobilized into the buffer for baseline.
4. Immerse the biosensor into the sample solution containing the antibody to be tested.
5. Immerse the sensor bound to the antibody to be tested in the buffer for dissociation.
6. Through the real-time monitoring of the thickness of the biosensor biofilm layer during the experiment, the kinetic constant of the sample to be tested can be obtained.