Analysis of Glycolate Oxidase Activity by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1368-LGZ)

Introduction

Sensitivity: 0.3 U/mL<br />Detection range: 0.3-350 U/mL<br />Precision: inter-lot difference of 8.199999999999999%, intra-lot difference of 1.1%<br />Detection equipment: Ultraviolet-visible Spectrophotometer<br />Detection wavelength: 324 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Glycolate oxidase catalyzes sodium glycolate substrate to produce glyoxylic acid, glyoxylic acid reacts with phenylhydrazine hydrochloride to produce phenylglyoxylic acid phenylhydrazone. The substance has an absorption peak at 324 nm, and its optical density is linear with the concentration of phenylhydrazone glyoxylate within a certain range. The amount of phenylhydrazone glyoxylate produced reflects the activity of glycolate oxidase.

Applications

It is used to detect the activity of glycolate oxidase in various plant tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: various plant tissues

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