Analysis of Lactate Dehydrogenase (LDH) Cytotoxicity by Enzyme-labeled Instrument (CAT#: STEM-MB-1447-LGZ)

Lactate dehydrogenase catalyzes the reaction of lactate and NAD+ to produce pyruvate and NADH. Under the action of PMS, NADH transfers electrons to WST-8 and produces yellow products with characteristic absorption peaks at 450 nm. Thus, the activity of lactate dehydrogenase can be quantified by colorimetry.

It can be used to detect the cytotoxicity of lactate dehydrogenase (LDH) release.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: cell sample