Analysis of Acid Phosphatase (ACP) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1396-LGZ)

Acid phosphatase can decompose phenylene disodium phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol reacts with 4-amino-antipyrine in alkaline solution and oxidizes to red quinone derivatives by potassium ferricyanide. The activity of enzyme can be measured according to the shade of red.

It is used to detect ACP activity of serum (plasma), pleural fluid, urine, cells, cell supernatant and various animal tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, urine