Analysis of Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1358-LGZ)

GSSG is reduced to GSH by glutathione reductase, and GSH can react with color substrate DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Total glutathione can then be calculated by measuring A412(absorbance at 412 nm). Then the GSH in the sample is removed with appropriate reagents and the GSSG content can be determined by using the reaction principle above.

It is suitable for the determination of total glutathione (T-GSH) and oxidized glutathione (GSSG) in serum (plasma), animal tissues, red blood cells and cultured cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), animal tissues, red blood cells and cultured cells