Analysis of Ascorbate Peroxidase (APX) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1329-LGZ)

Introduction

Sensitivity: 0.071 U/g<br />Detection range: 0.071-47 U/g<br />Precision: inter-lot difference of 6.4%, intra-lot difference of 4.8%<br />Detection equipment: Ultraviolet-visible Spectrophotometer<br />Detection wavelength: 290 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Ascorbic acid peroxidase (APX) catalyzes the reaction of ascorbic acid (ASA) with hydrogen peroxide (H2O2) to oxidize ASA into mono dehydroascorbic acid (MDASA). As ASA was oxidized, the absorbance value (A290) in the solution at 290nm wavelength decreased, and the ascorbate peroxidase (APX) activity was calculated according to the decrease value of A290 per unit time. ASA oxidation was calculated with extinction coefficient of 2.8 mL/ (μmol·cm).

Applications

It was used to detect the activity of ascorbate peroxidase in plant tissue.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: Plant tissue

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