Analysis of Sucrose by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1342-LGZ)

Sucrose in plant tissues is hydrolyzed to glucose and fructose by heating in a boiling water bath under acidic conditions. Fructose loses water under acidic conditions to produce 5-hydroxymethylfurfural, which results in ultraviolet absorption. Glucose must be dissimilated to the ketose structure and then dehydrated to produce 5-hydroxymethylfurfural, but glucose isomerizes to ketose very slowly. As a result, glucose produces much less UV absorption than fructose.

Used to detect sucrose content in plant tissues.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: plant tissues