Analysis of Glucose-6-Phosphate Dehydrogenase (G-6-PD) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1419-LGZ)

Introduction

Sensitivity: 0.01 U/L<br />Detection range: 0.01-50 U/L<br />Precision: inter-lot difference of 5.8%, intra-lot difference of 2.1%<br />Detection equipment: Enzyme-labeled Instrument<br />Detection wavelength: 450 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Glucose-6-phosphoric acid is oxidized to form 6-PG in the presence of G6PDH. In this reaction, NADP+ is reduced to NADPH. The resulting NADPH reduces WST-8 to form orange-yellow formazan with a maximum absorption peak at around 450 nm under the action of the electron-coupled reagent 1-mPMS. The formazan produced in the reaction system is directly proportional to the activity of G6DPH in the sample.

Applications

It is suitable for detecting the activity of glucose-6-phosphate dehydrogenase in animal tissue samples and serum (plasma).

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue samples and serum (plasma)

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