Analysis of D-Lactic Acid/Lactate(LA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1413-LGZ)

Using oxidized coenzyme Ⅰ (NAD+) as hydrogen receptor, D-lactate dehydrogenase (LDH) catalyzes the dehydrogenation of D-lactate to produce pyruvate, which converts NAD+ into NADH. N-methylphenazine methyl sulfate (PMS) dehydrogenated nitrotetrazazole blue (NBT) chloride to purple color, color absorbance at 530 nm and the content of lactic acid showed a linear relationship. During the whole process, L-lactic acid did not affect the determination result. Therefore, the content of D-lactic acid in the sample can be calculated by measuring the change of absorbance value at the wavelength 530 nm.

It is suitable for the determination of D-lactic acid in animal tissue samples and serum (plasma) samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples and serum (plasma) samples