Fluorescence immunoassay (FIA) to detect antigens and antibodys (CAT#: STEM-MB-0173-WXH)

Introduction

Fluorescence immunoassay is a sensitive technique that can be used in the measurement of many compounds, including drugs, hormones, and proteins; in the identification of antibodies; and in the quantification of antigens such as viral particles and, potentially, bacteria. The advantages of a Fluorescent detection system have been known for many years. These include higher sensitivity detection of the analyte, simplified reagents and simpler assay designs.

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Principle Applications Procedure Materials Notes Related Products

Principle

A modern fluorescent based immunoassay uses a fluorescent compound as the detection reagent which absorbs light or energy (excitation energy) at a specific wavelength and then emits light or energy at a different wavelength.

Applications

• Measurement of many compounds, including drugs, hormones, and proteins.
• Identification of antibodies.
• Quantification of antigens such as viral particles and, potentially, bacteria.

Procedure

1. Plate coated with mixed capture antibody.
2. Standard or samples incubated with the plated capture antibody.
3. Fluorescent detection antibody is added. After incubation and wash, fluorescent signal is measured with plate reader.

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