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Western blotting is a method for detecting a certain protein in a complex sample based on the specific combination of antigen and antibody. This method is a new immunobiochemical technology developed on the basis of gel electrophoresis and solid-phase immunoassay technology, high specificity and sensitivity.
The principle of Western blot: The cells or biological tissue samples treated by gel electrophoresis are stained by specific antibodies. Information about the expression of specific proteins in the analyzed cells or tissues can be obtained by analyzing the location and depth of staining.
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