Radioimmunoassay (RIA) to measure concentrations of substances (CAT#: STEM-MB-0171-WXH)

Introduction

Radioimmunoassay (RIA) technology is a new technology for in vitro determination of ultra-trace substances that combines the high sensitivity and accuracy of radioisotope measurement with the specificity of antigen-antibody reactions.<br />Radioimmunoassay generally uses competitive binding assay technology. The basic principle is that the radiolabeled antigen and the tested antigen (or standard substance) undergo a reversible competitive binding reaction to a limited specific antibody, and the amount of the finally formed radioactive antigen-antibody complex is negatively correlated with the content of the tested substance. When the amount of antibody is fixed, the formation of immune complexes is restricted by the amount of antigen to be tested. The advantages of the RIA method are sensitivity, specificity, simplicity, and small sample volume, and can often be measured to picomolar.