Equine Herpesvirus Type 2 Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0831-ZJF)

This kit utilizes SYBR Green dye-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Equine Herpesvirus Type 2 gene. In the presence of the target nucleic acid template, PCR amplification occurs, generating fluorescence signals. The fluorescence intensity is monitored in real time, enabling qualitative and quantitative analysis of Equine Herpesvirus Type 2 with high sensitivity and specificity.

Kit Components:
Equine Herpesvirus Type 2 Amplification Mix: 50 µL × 1 tube
Equine Herpesvirus Type 2 Reaction Mix: 950 µL × 1 tube
Equine Herpesvirus Type 2 Positive Control: 50 µL × 1 tube
Equine Herpesvirus Type 2 Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Designed for immediate use with user-provided DNA.
Selective primers crafted from highly conserved genetic regions.
Fine-tuned probe and primer chemistry enhances detection power.
Includes control sample for clear interpretation.
Sealed fluorescence PCR tube reduces contamination possibilities.
For investigational use in laboratories.

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