New Killer Francisella Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-2122-ZJF)

This kit utilizes probe-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the New Killer Francisella gene. The system enables in vitro amplification and detection of New Killer Francisella nucleic acids. When the target nucleic acid is present, the PCR reaction proceeds and generates fluorescence signals. These signals are monitored in real time by the instrument to provide both qualitative and quantitative results.

Kit Components:
New Killer Francisella Amplification Mix: 50 µL × 1 tube
New Killer Francisella Reaction Mix: 950 µL × 1 tube
New Killer Francisella Positive Control: 50 µL × 1 tube
New Killer Francisella Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Ready-to-use format; users only need to provide sample DNA.
Primers and probes are designed from conserved regions for high specificity.
Optimized oligo sequences deliver excellent sensitivity.
Positive control is included to help identify false-negative results.
Closed-tube, single-step workflow minimizes contamination risk.
Fast detection with minimal processing time.
For research use only.

STEM-SLSR-2120-ZJF
STEM-SLSR-2121-ZJF
STEM-SLSR-2123-ZJF
STEM-SLSR-2124-ZJF