Superresolution measurements in vivo: imaging Drosophila embryo by photoactivated localization microscopy (CAT#: STEM-MIT-0396-LJX)

Introduction

Drosophila often live on rotten fruit or in flowers. Its body size is small, the insect body is mostly yellow brown. Drosophila embryo refers to the young body which has the ability to develop into drosophila adult after several cell division and cell differentiation after the combination of male and female germ cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of photoactivated localization microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Applied in many areas of the life sciences

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Drosophila embryo

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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