Seplife LXPM Ni 5504 NTA Affinity Medium, ≥20 mg His Protein/mL (CAT#: STEM-BC-3123-ZJF)

This immobilized-metal affinity medium is designed for purification of His-tagged recombinant proteins and other metal-binding biomolecules. It uses a polymethyl methacrylate matrix with a hydrophilically modified surface. Chelating NTA groups coordinate nickel ions to form selective affinity-binding sites. Histidine residues on target proteins interact with the immobilized nickel during sample loading. The hydrophilic surface reduces nonspecific adsorption and supports product recovery. The 1000 Å pore structure permits access by proteins, peptides, nucleotides, and phosphorylated proteins. The rigid 80 µm particles support high flow and operation up to 0.8 MPa. The medium is compatible with selected denaturants, reducing agents, detergents, alcohols, acids, and alkaline solutions under the stated conditions.

Model: Seplife® LXPM Ni 5504 NTA
Matrix: Polymethyl methacrylate
Appearance: Light-blue opaque spherical particles
Chelating chemistry: NTA with Ni²⁺
Median particle size d50v: 80 µm
Pore size: 1000 Å
His-tagged protein capacity: ≥20 mg/mL
Recommended linear flow: 100-800 cm/h
Maximum pressure: 0.8 MPa
pH stability: 2-12
Storage solution: 20% ethanol with stated sodium chloride preservative
Chemical compatibility: 0.1 M sodium hydroxide, 0.1 M hydrochloric acid, 8 M urea, 6 M guanidine hydrochloride for 24 h, 30% isopropanol, 2% SDS, 5 mM DTT for 24 h, and 70% ethanol after nickel removal

NTA nickel affinity chemistry
≥20 mg/mL His-protein capacity
Low nonspecific adsorption
Hydrophilic particle surface
High-resolution affinity separation
Up to 800 cm/h flow
Denaturant-compatible operation
Rigid 0.8 MPa matrix

STEM-BC-0412-ZJF
STEM-BC-3074-ZJF
STEM-BC-3075-ZJF
STEM-BC-3078-ZJF
STEM-BC-3081-ZJF