Porcine Circovirus Type 2 Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1619-ZJF)

This kit utilizes probe-based real-time fluorescent PCR technology. Specific primers are designed to target highly conserved regions of the Porcine Circovirus Type 2 gene. The system enables in vitro amplification and detection of Porcine Circovirus Type 2 nucleic acids. When the target nucleic acid is present, the PCR reaction proceeds and generates fluorescence signals. These signals are monitored in real time by the instrument to provide both qualitative and quantitative results.

Kit Components:
Porcine Circovirus Type 2 Amplification Mix: 50 µL × 1 tube
Porcine Circovirus Type 2 Reaction Mix: 950 µL × 1 tube
Porcine Circovirus Type 2 Positive Control: 50 µL × 1 tube
Porcine Circovirus Type 2 Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

Immediate-use configuration simplifies workflow; DNA is the only input needed.
Sequence specificity ensured by probe design from conserved genomic areas.
Enhanced detection reliability via component refinement.
Includes internal positive standard for reference.
One-tube format reduces handling and contamination risks.
Quick runtime supports efficient processing.
Exclusively for research laboratories.

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