Mycoplasma agalactiae Probe-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-1750-ZJF)

This assay kit uses probe-based real-time PCR to amplify and detect Mycoplasma agalactiae. The workflow includes template amplification, signal generation, and real-time fluorescence tracking, delivering robust qualitative and quantitative data with high specificity and minimal background interference.

Kit Components:
Mycoplasma agalactiae Amplification Mix: 50 µL × 1 tube
Mycoplasma agalactiae Reaction Mix: 950 µL × 1 tube
Mycoplasma agalactiae Positive Control: 50 µL × 1 tube
Mycoplasma agalactiae Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: Reagents in the kit are sufficient for the number of tests indicated on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light, avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
Compatible with real-time PCR systems including ABI, MX3000P/3005P, LightCycler, Bio-Rad, and Eppendorf.
Additional required equipment: DNase/RNase-free 1.5 mL centrifuge tubes, 0.2 mL PCR reaction tubes, filter tips (10 µL, 200 µL, 1000 µL), centrifuge, vortex mixer, and pipettes of various capacities.
Sample Requirements:
Depending on circumstances, use intestinal tissue from dead or culled cattle under sterile conditions. For live animals, samples may include whole blood, serum, plasma, feces, vomit, oral/nasopharyngeal/respiratory secretions, throat/nasal/ocular/anal swabs, or viral cultures.
Cross-contamination must be avoided. Samples should be tested promptly after collection or stored at -20°C ± 5°C. For long-term storage, samples should be kept below -70°C.
Sample Storage and Transport:
Short-term: Store at -20°C.
Long-term (up to 6 months): Store below -70°C.
Transport using 2-8°C ice packs. Repeated freeze-thaw cycles are strictly prohibited.

All reagents pre-prepared; template DNA addition is the only step.
Probes and primers designed for precise recognition of target sequence.
High-sensitivity detection capability built into the system.
Includes positive control for experimental accuracy.
Closed-tube design reduces the chance of contamination.
Accelerated protocol supports time-saving diagnostics.
Only for use in research environments.

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