Ni Seplife FF TED Affinity Medium, ~25 µmol Ni²⁺/mL (CAT#: STEM-BC-3132-ZJF)

This nickel-chelating affinity medium is designed for purification of His-tagged recombinant proteins. It uses a high-flow agarose matrix with TED chelating chemistry. The ligand coordinates nickel ions strongly to reduce metal leakage during operation. Histidine residues on target proteins interact selectively with the immobilized nickel. The stable chelation permits purification in the presence of specified EDTA and DTT concentrations. The medium can also process compatible peptides, proteins, nucleotides, and phosphorylated proteins. Spherical particles provide uniform packing and consistent separation performance. High flow capability supports rapid processing with relatively low backpressure. Broad pH stability enables repeated cleaning-in-place and reuse.

Model: Ni Seplife FF(TED)
Appearance: Spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Chelating chemistry: TED with Ni²⁺
Particle size: 45-165 µm
Maximum flow rate: ≥370 cm/h
Pressure resistance: 0.3 MPa
Nickel-ion binding amount: Approximately 25 µmol/mL
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 0.01 M sodium hydroxide, 0.01 M hydrochloric acid, 6 M guanidine hydrochloride for 24 h, 1 M sodium hydroxide for 24 h, 10 mM EDTA for 24 h, 100 mM EDTA for 2 h, and 5 mM DTT for 24 h

Stable TED nickel chelation
EDTA-compatible purification
DTT-tolerant operation
Selective His-tag binding
≥370 cm/h flow
Low metal-ion leakage
Uniform agarose particles
Broad CIP pH range

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