Ni Seplife FF IDA Affinity Medium, ~30 µmol Ni²⁺/mL (CAT#: STEM-BC-3131-ZJF)

This immobilized-metal affinity chromatography medium is designed for purification of His-tagged recombinant proteins. It uses a high-flow agarose matrix functionalized with iminodiacetic acid groups. The IDA ligand chelates nickel ions to form affinity-binding sites for histidine residues. The medium can also purify compatible peptides, proteins, nucleotides, and phosphorylated proteins. Spherical particles support uniform column packing and reproducible chromatographic performance. The high-flow agarose structure permits rapid processing with relatively low backpressure. Broad pH stability supports repeated purification and cleaning-in-place cycles. The medium tolerates the listed denaturants, alkaline solutions, and reducing-agent conditions. Its chemical stability and biocompatibility support routine downstream bioprocessing.

Model: Ni Seplife FF(IDA)
Appearance: Spherical gel, odorless and tasteless
Matrix: Seplife 6FF
Chelating ligand: Iminodiacetic acid
Functional ion: Ni²⁺
Particle size: 45-165 µm
Maximum flow rate: ≥370 cm/h
Pressure resistance: 0.3 MPa
Nickel-ion binding amount: Approximately 30 µmol/mL
Long-term pH stability: 3-13
Short-term CIP pH stability: 2-14
Chemical compatibility: 0.1 M sodium hydroxide, 8 M urea for 24 h, 6 M guanidine hydrochloride for 24 h, 1 M sodium hydroxide for 24 h, and 5 mM DTT for 24 h

IDA nickel-chelating chemistry
Selective histidine binding
≥370 cm/h flow capability
Uniform spherical particles
Broad pH stability
Denaturant-compatible operation
Reducing-agent tolerance
Reproducible affinity purification

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