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Measurement of Cell Dynamics by Dynamic light scattering (DLS) (CAT#: STEM-MB-0546-WXH)
Introduction
Cellular Dynamics studies the dynamics reaction shown by the cell during various processes. In cellular dynamcis the reaction shown by the components of the cell during various process such as cell differentiaion and implications on cell functions are studied in cellular dynamics.
Dynamic light scattering (DLS) is used to study dynamic characteristics and it has the potential to measure cell dynamics.
Principle
Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
Applications
DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.
Procedure
1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis
2. Measurement by DLS instrument
3. Data analysis
Materials
• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector
• Dynamic Light Scattering Detector
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