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Study of solution conformation and dynamics of superhelical DNA by Dynamic light scattering (DLS) (CAT#: STEM-MB-0554-WXH)
Introduction
Dynamic light scattering is a powerful tool for studying the shape and internal motions of flexible biopolymers, such as DNA. For superhelical DNA at small scattering angles, the rigid rod model can be applied with good success to obtain the translational and rotational diffusion coefficients of the molecule.
Principle
Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
Applications
DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.
Procedure
1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis
2. Measurement by DLS instrument
3. Data analysis
Materials
• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector
• Dynamic Light Scattering Detector
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