High-quality products
Reliable performance
- Home
- Technical Services
- Spectroscopy Technology
- Dynamic and Static Light Scattering (DLS / SLS) Technology
- Dynamic Light Scattering (DLS) Technology
Molecular weight determination by Dynamic light scattering (DLS) (CAT#: STEM-MB-0544-WXH)
Introduction
Molecular weight is one of the most important properties of any molecule. Comparison of sequence molecular weight of proteins or nucleic acids with experimentally determined molecular weight can provide useful information such as their oligomeric status, degradation, or change in molecular weight upon interaction between binding partners.
Principle
Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.
Applications
DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.
Procedure
1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis
2. Measurement by DLS instrument
3. Data analysis
Materials
• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector
• Dynamic Light Scattering Detector
Other recommended products
-
-
Characterization of PKR–RNA complex by Dynamic light scattering (DLS) (CAT#: STEM-MB-0542-WXH)
-
Homogeneity studies of Aprotinin v (CAT#: STEM-MB-0538-WXH)
-
Characterize the size of vesicles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0557-WXH)
-
Measurement of Cell Dynamics by Dynamic light scattering (DLS) (CAT#: STEM-MB-0546-WXH)
-
Characterize the size of virus-like particles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0556-WXH)
-
Characterization of OAS1– RNA complex by Dynamic light scattering (DLS) (CAT#: STEM-MB-0541-WXH)
-
Homogeneity studies of Carbonic anhydrase by Dynamic light scattering (DLS) (CAT#: STEM-MB-0537-WXH)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping