Molecular weight determination by Dynamic light scattering (DLS) (CAT#: STEM-MB-0544-WXH)

Introduction

Molecular weight is one of the most important properties of any molecule. Comparison of sequence molecular weight of proteins or nucleic acids with experimentally determined molecular weight can provide useful information such as their oligomeric status, degradation, or change in molecular weight upon interaction between binding partners.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

Other recommended products

Identifying the interactions during protein refolding reactions by Dynamic light scattering (DLS) (CAT#: STEM-MB-0553-WXH)
Characterization of PKR–RNA complex by Dynamic light scattering (DLS) (CAT#: STEM-MB-0542-WXH)
Measurement of Cell Dynamics by Dynamic light scattering (DLS) (CAT#: STEM-MB-0546-WXH)
Characterize the size of micelles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0555-WXH)
Homogeneity studies of Ferritin by Dynamic light scattering (DLS) (CAT#: STEM-MB-0534-WXH)
Homogeneity studies of ovalbumin by Dynamic light scattering (DLS) (CAT#: STEM-MB-0532-WXH)
Nanosize Particle Analysis by Dynamic light scattering (DLS) by Dynamic light scattering (DLS) (CAT#: STEM-MB-0548-WXH)
Tracing protein aggregates by Dynamic light scattering (DLS) (CAT#: STEM-MB-0549-WXH)