ESBL-Producing Klebsiella Pneumoniae Dye-Based qPCR Kit, 50 Tests/Box (CAT#: STEM-SLSR-0823-ZJF)

Designed for the detection of ESBL-Producing Klebsiella Pneumoniae, this kit employs SYBR Green-based real-time PCR. It uses highly specific primers to amplify conserved gene regions of the target sequence. The real-time detection system monitors fluorescence generated during amplification, allowing for accurate qualitative and quantitative evaluation of ESBL-Producing Klebsiella Pneumoniae in various biological or food samples.

Kit Components:
ESBL-Producing Klebsiella Pneumoniae Amplification Mix: 50 µL × 1 tube
ESBL-Producing Klebsiella Pneumoniae Reaction Mix: 950 µL × 1 tube
ESBL-Producing Klebsiella Pneumoniae Positive Control: 50 µL × 1 tube
ESBL-Producing Klebsiella Pneumoniae Negative Control: 50 µL × 1 tube
Instruction Manual: 1 copy
Note 1: Components from different lot numbers must not be used interchangeably.
Note 2: The kit components are sufficient for the number of tests specified on the label.
Storage Conditions and Shelf Life:
-20°C ± 5°C, protect from light; avoid repeated freeze-thaw cycles (less than 7 times); shelf life is 12 months.
Applicable Instruments:
1.5 mL DNase/RNase-free centrifuge tubes
0.2 mL PCR reaction tubes
Filter tips (specifications: 10 µL, 200 µL, 1000 µL)
Centrifuge, vortex mixer, micropipettes of various specifications
Agarose, electrophoresis tank, power supply, gel imaging system
Sample Requirements:
Acceptable sample types include food, feces, vomit, oral samples, saliva, nasal/throat/eye/anal swabs, and virus cultures.
Cross-contamination should be avoided. Samples should be tested immediately after collection or stored at -20°C ± 5°C. For long-term storage, keep below -70°C.
Sample Storage and Transport:
Samples can be stored short-term at -20°C.
For long-term storage, keep below -70°C (no longer than 6 months).
During transportation, use 2-8°C ice packs. Repeated freeze-thawing is strictly prohibited.

Simplified protocol: only DNA needs to be added.
High-precision primers designed for analyte-specific detection.
Reagents optimized for robust signal sensitivity.
Positive control supports result validation and QC.
Fully enclosed PCR setup mitigates contamination risks.
Limited to academic or laboratory research.

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